Summary of Study ST003897

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002438. The data can be accessed directly via it's Project DOI: 10.21228/M8H53D This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003897
Study TitlePostprandial Plasma Lipidomic Changes in 147 Individuals Following Ingestion of a Standard Mixed Meal
Study SummaryUsing untargeted lipidomics, this study analyzed postprandial plasma lipid profile changes in 147 participants after consumption of a standard mixed meal. Time-series analyses revealed dynamic responses in multiple key metabolic pathways during the postprandial state and explored associations between metabolite trajectories and individual metabolic health status. The findings showed inter-individual variability in both the magnitude and pattern of postprandial metabolite changes, underscoring the importance of personalized metabolism.We found that substances such as TG 50:4|TG 16:0_16:1_18:3, PE P-38:6|PE P-18:1_20:5, PC 38:6, CAR 10:0, DG 51:12, and SM 34:1;2O showed significant changes after the standard mixed meal test, and were highly correlated with hormone fluctuations including Insulin and Glucagon.
Institute
Nanjing Medical University
Last NameWang
First NameJiachen
AddressNo. 300, Guangzhou Road, Nanjing
Emailjcwang@stu.njmu.edu.cn
Phone+86 02565306466
Submit Date2025-04-27
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailOther
Release Date2025-07-30
Release Version1
Jiachen Wang Jiachen Wang
https://dx.doi.org/10.21228/M8H53D
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002438
Project DOI:doi: 10.21228/M8H53D
Project Title:Postprandial Plasma Multi-Omics Changes in Response to a Standard Mixed Meal or Individual Macronutrients in Humans
Project Summary:Postprandial metabolism is a complex and dynamic physiological process involving numerous metabolic pathways and biomolecular interactions. It plays a critical role in the development of chronic diseases such as obesity, diabetes, and cardiovascular disease. This study employed an interventional design in human participants to systematically compare the dynamic postprandial changes in plasma multi-omics profiles following ingestion of a standard mixed meal versus four individual macronutrients (glucose, protein, butter, and olive oil). The aim is to elucidate the specific effects of different nutrients on human postprandial metabolic networks and their potential implications for health, thereby providing foundational data to support personalized nutrition strategies and disease prevention.
Institute:Nanjing Medical University
Last Name:Wang
First Name:Jiachen
Address:No. 300, Guangzhou Road, Nanjing
Email:jcwang@stu.njmu.edu.cn
Phone:+86 02565306466

Subject:

Subject ID:SU004032
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time
SA429097P103A0
SA429098P97A0
SA429099P98A0
SA429100P99A0
SA429101P100A0
SA429102P101A0
SA429103P102A0
SA429104P104A0
SA429105P95A0
SA429106P105A0
SA429107P106A0
SA429108P107A0
SA429109P108A0
SA429110P109A0
SA429111P110A0
SA429112P96A0
SA429113P94A0
SA429114P112A0
SA429115P84A0
SA429116P78A0
SA429117P79A0
SA429118P80A0
SA429119P81A0
SA429120P82A0
SA429121P83A0
SA429122P85A0
SA429123P93A0
SA429124P86A0
SA429125P87A0
SA429126P88A0
SA429127P89A0
SA429128P90A0
SA429129P91A0
SA429130P92A0
SA429131P111A0
SA429132P113A0
SA429133P76A0
SA429134P140A0
SA429135P134A0
SA429136P135A0
SA429137P136A0
SA429138P137A0
SA429139P138A0
SA429140P139A0
SA429141P141A0
SA429142P132A0
SA429143P142A0
SA429144P143A0
SA429145P144A0
SA429146P145A0
SA429147P146A0
SA429148P147A0
SA429149P2A0
SA429150P133A0
SA429151P131A0
SA429152P114A0
SA429153P121A0
SA429154P115A0
SA429155P116A0
SA429156P117A0
SA429157P118A0
SA429158P119A0
SA429159P120A0
SA429160P122A0
SA429161P130A0
SA429162P123A0
SA429163P124A0
SA429164P125A0
SA429165P126A0
SA429166P127A0
SA429167P128A0
SA429168P129A0
SA429169P77A0
SA429170P1A0
SA429171P75A0
SA429172P28A0
SA429173P74A0
SA429174P23A0
SA429175P24A0
SA429176P25A0
SA429177P26A0
SA429178P27A0
SA429179P29A0
SA429180P20A0
SA429181P30A0
SA429182P31A0
SA429183P32A0
SA429184P33A0
SA429185P34A0
SA429186P35A0
SA429187P21A0
SA429188P19A0
SA429189P37A0
SA429190P9A0
SA429191P3A0
SA429192P4A0
SA429193P5A0
SA429194P6A0
SA429195P7A0
SA429196P8A0
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Collection:

Collection ID:CO004025
Collection Summary:Participants venous blood samples were collected at baseline (0 min) and at 30 min, 60 min, 120 min, and 180 min post-meal and stored at -80 C until analysis.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR004041
Treatment Summary:Volunteers were recruited in Nanjing, China, via poster advertisements to participate in a standard MMTT (Mixed Meal Tolerance Test). The inclusion criteria were as follows: age ≥ 18 years old, no prior diagnosis of diabetes; no history of malignant tumors, severe organ disease, or significant acute or chronic infections; and no recent use of glucocorticoids, immunosuppressants, or other medications known to substantially affect metabolism. A total of 147 participants (77 males and 70 females, aged 18-50 years) completed the MMTT. Among them, 54 participants were of normal weight (BMI ≤ 24 kg/m²), 38 were overweight (24 kg/m² < BMI ≤ 28 kg/m²), and 55 were categorised as obese (BMI > 28 kg/m²). We utilized Ensure powder (Abbott Manufacturing Singapore Private Limited), a standardized nutritional meal replacement, which provides 57.4g of carbohydrates, 15.9g of protein, and 14g of fat per 100g, delivering 430 kcal of energy. After fasting for 12 hours, each participant was instructed to consume 84g of Ensure (360 kcal) dissolved in 250 ml of water within 3-5 minutes. Participants venous blood samples were collected at baseline (0 min) and at 30 min, 60 min, 120 min, and 180 min post-meal.

Sample Preparation:

Sampleprep ID:SP004038
Sampleprep Summary:Ice-cold methanol and methyl tert-butyl ether (MTBE, Optima brand, Fisher Scientific) was sequentially added into plasma sample, mixture was incubated at room temperature for 30 min. Phase separation was induced by adding and incubation with MS-grade water at room temperature for 10 min, and centrifuged at 1,000 g for 10 min. The upper organic phase was collected and dried in a vacuum centrifuge. All non-polar extracts were resuspended in 75% isopropanol (IPA) in water. The temperature of the autosampler during LC-MS analyses were stored at 4°C. Samples were vortexed, centrifuged (21,000g, 20 min, 4°C) and the supernatant was aliquoted into a HPLC vial for the LC-MS analysis. Metabolic extracts were separated on BEH C18 column (Waters, SKU: 186002352, 100 mm × 2.1 mm, 1.7 μm) using a 20-min gradient with organic phase increased from 30% to 100% at 40℃(solvent A: 0.1% formic acid, 2 mM ammonium formate in 60/40 acetonitrile/water; solvent B: 2 mM ammonium formate in 90/10 IPA/acetonitrile). LC-MS analyses were performed as described above in the metabolomics section.

Chromatography:

Chromatography ID:CH004852
Chromatography Summary:DDA lipidomics analysis was performed using a Thermo Vanquish (Thermo Fisher Scientific) with Q Exative Plus orbitrap high-resolution mass spectrometry (Thermo Fisher Scientific, USA), data acquisition software XCalibur 4.3 (Thermo Fisher Scientific). The main parameters are as follows. C18 detection mode: mobile phase was water: ACN(40:60)/ IPA: ACN(90:10), solvent was added with 0.1% FA and 2 mM ammonium formate, AcquityTM BEH C18 column (Waters Co., USA, 1.7 μm, 2.1×100 mm), separation gradient: the organic phase was increased from 30% to 100% in 20 min, and the remaining 5 min was used to flush and equilibrate the column; flow rate: 0.3 mL/min, injection volume: 5 μL, column temperature: 40 °C. The detection was carried out in positive and negative ion modes, respectively. Mass spectrometer: spray voltage: 3.2 kV(+) and 3.0 KV(-); capillary temperature: 350 °C; S-lens: 50%; collision energy: 20 %+30 % HCD; sheath gas flow: 40 arb, auxiliary gas flow rate: 10 arb, ; fragmentation gas: ultra-pure nitrogen, resolution setting: first level 70,000@m/z 200, two Level 17,500@m/z 200; Max IT: full MS 100 ms, full MS/MS 50 ms; parent ion scanning range: m/z 200-1800.
Instrument Name:Waters Acquity
Column Name:Waters ACQUITY UPLC CSH C18 (150 x 2.1mm,1.7um)
Column Temperature:40
Flow Gradient:the organic phase (B) was increased from 30% to 100% in 20 min, and the remaining 5 min was used to flush and equilibrate the column
Flow Rate:0.3 mL/min
Solvent A:40% water/60% acetonitrile
Solvent B:90% isopropanol/10% acetonitrile
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006400
Analysis Type:MS
Chromatography ID:CH004852
Num Factors:3
Num Metabolites:436
Units:Normalized counts
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