Summary of Study ST003942

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002469. The data can be accessed directly via it's Project DOI: 10.21228/M8H26H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST003942
Study TitleTryptophan metabolite concentrations in plasma of treated metabolic syndrome patients with and without mild cognitive impairment
Study SummaryWe validated mass spectrometry-based quantitation methods and quantified trytophan metabolites in kynurenine pathway in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment.
Institute
Mahidol University
DepartmentFaculty of Medicine Siriraj Hospital
LaboratorySiCORE-MSB
Last NameJariyasopit
First NameNarumol
Address2 Prannok, Bangkok, Non-US, 10700, Thailand
Emailnarumoljariyasopit@gmail.com
Phone+6624195500
Submit Date2025-05-28
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Waters)
Analysis Type DetailLC-MS
Release Date2025-06-25
Release Version1
Narumol Jariyasopit Narumol Jariyasopit
https://dx.doi.org/10.21228/M8H26H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002469
Project DOI:doi: 10.21228/M8H26H
Project Title:Higher Plasma Kynurenine to Tryptophan Correlates with Increased Incidence of Mild Cognitive Impairment in Treated Metabolic Syndrome Patients
Project Type:MS quantitative analysis
Project Summary:An increase in cognitive impairment has been observed in metabolic syndrome (MetS) patients. Although alterations in metabolomic profiles have been identified as potential plasma/serum biomarkers of mild cognitive impairment (MCI) and MetS, findings remain inconsistent— likely due to the heterogeneity among MetS patients and the lack of subsequent validation using targeted analysis after initial untargeted analysis. In this study, we validated mass spectrometry-based quantitation methods and quantified amino acids, fatty acids, and tryptophan metabolites in the kynurenine pathway in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment. We found that MCI was positively associated with kynurenine to tryptophan ratio (KTR) after the adjustment for age, gender, and BMI, as well as were negatively associated with C20:3 [all-Z-8,11,14] and lysine. One-unit increase in KTR resulted in increased probability of developing MCI by 371%. In contrast, one-unit increases in C20:3 and lysine were associated with decreased odds of developing MCI by 81% and 78%, respectively. Our finding underscores the prominent neuroinflammation, beyond normal aging, in MetS patients, even under ongoing clinical treatment. It also points to the potential of KTR as a risk marker for MCI, offering a valuable complement to the existing cognitive assessments that may be influenced by educational background. In addition, the validated metabolite data is an useful resource for future research. It can facilitate comparisons across different studies, contribute to large-scale analyses, and be used in machine learning models for discovering and validating new biomarkers.
Institute:Mahidol University
Department:Faculty of Medicine Siriraj Hospital
Laboratory:SiCORE-MSB
Last Name:Jariyasopit
First Name:Narumol
Address:2 Prannok, Bangkok, Non-US, 10700, Thailand
Email:narumoljariyasopit@gmail.com
Phone:662-4195507

Subject:

Subject ID:SU004079
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Human Ethnicity:Thai

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA45052931aug23_050plasma MetS
SA45053031aug23_095plasma MetS
SA45053131aug23_091plasma MetS
SA45053231aug23_042plasma MetS
SA45053331aug23_043plasma MetS
SA45053431aug23_047plasma MetS
SA45053531aug23_048plasma MetS
SA45053631aug23_051plasma MetS
SA45053731aug23_101plasma MetS
SA45053831aug23_055plasma MetS
SA45053931aug23_083plasma MetS
SA45054031aug23_082plasma MetS
SA45054131aug23_059plasma MetS
SA45054231aug23_060plasma MetS
SA45054331aug23_063plasma MetS
SA45054431aug23_066plasma MetS
SA45054531aug23_096plasma MetS
SA45054631aug23_038plasma MetS
SA45054731aug23_028plasma MetS
SA45054831aug23_104plasma MetS
SA45054931aug23_015plasma MetS
SA45055031aug23_021plasma MetS
SA45055131aug23_017plasma MetS
SA45055231aug23_026plasma MetS
SA45055331aug23_007plasma MetS
SA45055431aug23_079plasma MetS-MCI
SA45055531aug23_078plasma MetS-MCI
SA45055631aug23_116plasma MetS-MCI
SA45055731aug23_080plasma MetS-MCI
SA45055831aug23_086plasma MetS-MCI
SA45055931aug23_115plasma MetS-MCI
SA45056031aug23_114plasma MetS-MCI
SA45056131aug23_076plasma MetS-MCI
SA45056231aug23_113plasma MetS-MCI
SA45056331aug23_112plasma MetS-MCI
SA45056431aug23_084plasma MetS-MCI
SA45056531aug23_085plasma MetS-MCI
SA45056631aug23_081plasma MetS-MCI
SA45056731aug23_087plasma MetS-MCI
SA45056831aug23_111plasma MetS-MCI
SA45056931aug23_102plasma MetS-MCI
SA45057031aug23_109plasma MetS-MCI
SA45057131aug23_107plasma MetS-MCI
SA45057231aug23_090plasma MetS-MCI
SA45057331aug23_106plasma MetS-MCI
SA45057431aug23_105plasma MetS-MCI
SA45057531aug23_092plasma MetS-MCI
SA45057631aug23_093plasma MetS-MCI
SA45057731aug23_073plasma MetS-MCI
SA45057831aug23_094plasma MetS-MCI
SA45057931aug23_103plasma MetS-MCI
SA45058031aug23_097plasma MetS-MCI
SA45058131aug23_099plasma MetS-MCI
SA45058231aug23_100plasma MetS-MCI
SA45058331aug23_074plasma MetS-MCI
SA45058431aug23_005plasma MetS-MCI
SA45058531aug23_072plasma MetS-MCI
SA45058631aug23_020plasma MetS-MCI
SA45058731aug23_032plasma MetS-MCI
SA45058831aug23_031plasma MetS-MCI
SA45058931aug23_030plasma MetS-MCI
SA45059031aug23_029plasma MetS-MCI
SA45059131aug23_025plasma MetS-MCI
SA45059231aug23_023plasma MetS-MCI
SA45059331aug23_022plasma MetS-MCI
SA45059431aug23_019plasma MetS-MCI
SA45059531aug23_034plasma MetS-MCI
SA45059631aug23_018plasma MetS-MCI
SA45059731aug23_016plasma MetS-MCI
SA45059831aug23_014plasma MetS-MCI
SA45059931aug23_012plasma MetS-MCI
SA45060031aug23_010plasma MetS-MCI
SA45060131aug23_009plasma MetS-MCI
SA45060231aug23_008plasma MetS-MCI
SA45060331aug23_033plasma MetS-MCI
SA45060431aug23_035plasma MetS-MCI
SA45060531aug23_071plasma MetS-MCI
SA45060631aug23_057plasma MetS-MCI
SA45060731aug23_070plasma MetS-MCI
SA45060831aug23_069plasma MetS-MCI
SA45060931aug23_068plasma MetS-MCI
SA45061031aug23_065plasma MetS-MCI
SA45061131aug23_062plasma MetS-MCI
SA45061231aug23_006plasma MetS-MCI
SA45061331aug23_058plasma MetS-MCI
SA45061431aug23_056plasma MetS-MCI
SA45061531aug23_036plasma MetS-MCI
SA45061631aug23_054plasma MetS-MCI
SA45061731aug23_053plasma MetS-MCI
SA45061831aug23_049plasma MetS-MCI
SA45061931aug23_044plasma MetS-MCI
SA45062031aug23_041plasma MetS-MCI
SA45062131aug23_040plasma MetS-MCI
SA45062231aug23_039plasma MetS-MCI
SA45062331aug23_061plasma MetS-MCI
Showing results 1 to 95 of 95

Collection:

Collection ID:CO004072
Collection Summary:Blood was collected in EDTA-coated tubes and centrifuged at 3,000 rpm to obtain plasma. The plasma was then transferred into cryovials and stored at –85°C until use.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR004088
Treatment Summary:All patients were divided into two groups: 1) MetS patients with MCI and 2) MetS patients with normal cognitive function. MCI was determined when the MoCA score was less than 23.

Sample Preparation:

Sampleprep ID:SP004085
Sampleprep Summary:Tryptophan metabolites were quantified using a previously published method with minor modifications. In brief, 50 µL of plasma was mixed with 200 µL of methanol containing 100 ng of picolinic acid-d4, used as an internal standard. After a brief vortex, the sample mixture was sonicated for 10 min at room temperature and stored at -20ºC overnight. The sample was centrifuged at 13,000 rpm at 4ºC for 15 min. The supernatant was transferred to a new Eppendorf tube and evaporated to dryness using a vacuum concentrator (Labconco, MO, USA). The sample was resuspended in 100 µL Milli-Q water with 0.1% formic acid, followed by a brief vortex and 10-minute sonication at room temperature. After centrifugation at 13,000 rpm, 4ºC for 15 min, the supernatant was transfer to an LC vial.

Chromatography:

Chromatography ID:CH004923
Chromatography Summary:The analysis was carried out using a Waters Acquity I-Class UPLC coupled with a Xevo TQ-Absolute MS/MS, with an electrospray ionization source. The target tryptophan metabolites were separated on a HSS T3 column, 2.1 × 100 mm, 1.8 mM column (Waters, Milford, MA, USA). The column was kept at 30ºC, and the flow rate was 0.3 mL/min throughout the analysis. Mobile phases were (A) 0.1% formic acid in Milli-Q water and (B) 0.1% formic acid in acetonitrile, using a gradient program that started at 99% A, decreased 30to 70% over 7.0 min, then decreased to 30% at 9 min, and returned to the initial condition at 10 min and held for 4.0 min. The MS was operated in positive multiple reaction monitoring mode to collect m/z values as given in the previous study.31 The optimal MS parameters were as follows: capillary voltage 1.5 kV, source temperature 150ºC, desolvation gas (N2) flow 900 L/h at 550 ºC, cone gas flow 150 L/h, nebulizer gas (Ar) 7.0 bar, collision gas flow 0.25 mL/min. The injection volume was 5 µL.
Instrument Name:Waters Acquity I-Class
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1 mm, 1.8 µm)
Column Temperature:30ºC
Flow Gradient:n/a
Flow Rate:0.3 mL/min
Solvent A:100% Water; 0.1% Formic acid
Solvent B:100% Acetonitrile; 0.1% Formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006481
Analysis Type:MS
Chromatography ID:CH004923
Num Factors:2
Num Metabolites:5
Units:µmol/dL plasma
  logo