Summary of Study ST003946

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002469. The data can be accessed directly via it's Project DOI: 10.21228/M8H26H This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST003946
Study TitlePlasma tryptophan concentrations in treated metabolic syndrome patients with and without mild cognitive impairment
Study SummaryWe validated mass spectrometry-based quantitation methods and quantified tryptophan in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment.
Institute
Mahidol University
DepartmentFaculty of Medicine Siriraj Hospital
LaboratorySiCORE-MSB
Last NameJariyasopit
First NameNarumol
Address2 Prannok, Bangkok, Non-US, 10700, Thailand
Emailnarumoljariyasopit@gmail.com
Phone+6624195500
Submit Date2025-05-28
Raw Data AvailableYes
Raw Data File Type(s)mzML, raw(Thermo)
Analysis Type DetailLC-MS
Release Date2025-06-25
Release Version1
Narumol Jariyasopit Narumol Jariyasopit
https://dx.doi.org/10.21228/M8H26H
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002469
Project DOI:doi: 10.21228/M8H26H
Project Title:Higher Plasma Kynurenine to Tryptophan Correlates with Increased Incidence of Mild Cognitive Impairment in Treated Metabolic Syndrome Patients
Project Type:MS quantitative analysis
Project Summary:An increase in cognitive impairment has been observed in metabolic syndrome (MetS) patients. Although alterations in metabolomic profiles have been identified as potential plasma/serum biomarkers of mild cognitive impairment (MCI) and MetS, findings remain inconsistent— likely due to the heterogeneity among MetS patients and the lack of subsequent validation using targeted analysis after initial untargeted analysis. In this study, we validated mass spectrometry-based quantitation methods and quantified amino acids, fatty acids, and tryptophan metabolites in the kynurenine pathway in plasma of ninety-five treated MetS patients with and without MCI assessed by Montreal cognitive assessment. We found that MCI was positively associated with kynurenine to tryptophan ratio (KTR) after the adjustment for age, gender, and BMI, as well as were negatively associated with C20:3 [all-Z-8,11,14] and lysine. One-unit increase in KTR resulted in increased probability of developing MCI by 371%. In contrast, one-unit increases in C20:3 and lysine were associated with decreased odds of developing MCI by 81% and 78%, respectively. Our finding underscores the prominent neuroinflammation, beyond normal aging, in MetS patients, even under ongoing clinical treatment. It also points to the potential of KTR as a risk marker for MCI, offering a valuable complement to the existing cognitive assessments that may be influenced by educational background. In addition, the validated metabolite data is an useful resource for future research. It can facilitate comparisons across different studies, contribute to large-scale analyses, and be used in machine learning models for discovering and validating new biomarkers.
Institute:Mahidol University
Department:Faculty of Medicine Siriraj Hospital
Laboratory:SiCORE-MSB
Last Name:Jariyasopit
First Name:Narumol
Address:2 Prannok, Bangkok, Non-US, 10700, Thailand
Email:narumoljariyasopit@gmail.com
Phone:662-4195507

Subject:

Subject ID:SU004083
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606
Gender:Male and female
Human Ethnicity:Thai

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Sample source Factor
SA45092829aug23_103plasma MetS
SA45092929aug23_039plasma MetS
SA45093030aug23_004plasma MetS
SA4509311Sep23_015plasma MetS
SA45093229aug23_096plasma MetS
SA45093330aug23_012plasma MetS
SA45093429aug23_115plasma MetS
SA45093529aug23_063plasma MetS
SA45093629aug23_091plasma MetS
SA45093729aug23_111plasma MetS
SA45093829aug23_077plasma MetS
SA45093929aug23_051plasma MetS
SA45094029aug23_056plasma MetS
SA45094129aug23_101plasma MetS
SA45094229aug23_067plasma MetS
SA45094329aug23_095plasma MetS
SA45094429aug23_099plasma MetS
SA45094529aug23_118plasma MetS
SA4509461Sep23_011plasma MetS
SA45094729aug23_107plasma MetS
SA45094829aug23_079plasma MetS
SA45094929aug23_112plasma MetS
SA45095029aug23_098plasma MetS
SA45095129aug23_068plasma MetS
SA45095229aug23_057plasma MetS
SA45095329aug23_109plasma MetS-MCI
SA45095429aug23_075plasma MetS-MCI
SA45095530aug23_007plasma MetS-MCI
SA45095629aug23_069plasma MetS-MCI
SA45095729aug23_122plasma MetS-MCI
SA45095830aug23_011plasma MetS-MCI
SA45095929aug23_110plasma MetS-MCI
SA45096029aug23_088plasma MetS-MCI
SA45096129aug23_040plasma MetS-MCI
SA45096229aug23_072plasma MetS-MCI
SA45096330aug23_002plasma MetS-MCI
SA45096429aug23_045plasma MetS-MCI
SA45096529aug23_052plasma MetS-MCI
SA45096630aug23_013plasma MetS-MCI
SA45096729aug23_123plasma MetS-MCI
SA45096829aug23_106plasma MetS-MCI
SA45096929aug23_119plasma MetS-MCI
SA45097029aug23_041plasma MetS-MCI
SA45097129aug23_117plasma MetS-MCI
SA4509721Sep23_017plasma MetS-MCI
SA45097329aug23_113plasma MetS-MCI
SA45097429aug23_060plasma MetS-MCI
SA45097529aug23_105plasma MetS-MCI
SA45097629aug23_092plasma MetS-MCI
SA45097729aug23_108plasma MetS-MCI
SA45097830aug23_009plasma MetS-MCI
SA45097929aug23_064plasma MetS-MCI
SA45098029aug23_071plasma MetS-MCI
SA45098129aug23_121plasma MetS-MCI
SA4509821Sep23_016plasma MetS-MCI
SA45098329aug23_120plasma MetS-MCI
SA45098429aug23_065plasma MetS-MCI
SA45098529aug23_125plasma MetS-MCI
SA45098630aug23_006plasma MetS-MCI
SA45098729aug23_084plasma MetS-MCI
SA45098829aug23_058plasma MetS-MCI
SA45098929aug23_114plasma MetS-MCI
SA45099029aug23_043plasma MetS-MCI
SA45099129aug23_061plasma MetS-MCI
SA45099229aug23_087plasma MetS-MCI
SA45099329aug23_082plasma MetS-MCI
SA45099429aug23_097plasma MetS-MCI
SA45099529aug23_073plasma MetS-MCI
SA45099629aug23_053plasma MetS-MCI
SA45099729aug23_047plasma MetS-MCI
SA4509981Sep23_013plasma MetS-MCI
SA45099929aug23_093plasma MetS-MCI
SA4510001Sep23_018plasma MetS-MCI
SA4510011Sep23_014plasma MetS-MCI
SA45100229aug23_038plasma MetS-MCI
SA45100329aug23_083plasma MetS-MCI
SA45100429aug23_085plasma MetS-MCI
SA4510051Sep23_012plasma MetS-MCI
SA45100629aug23_100plasma MetS-MCI
SA45100729aug23_094plasma MetS-MCI
SA45100829aug23_086plasma MetS-MCI
SA45100929aug23_070plasma MetS-MCI
SA45101029aug23_089plasma MetS-MCI
SA45101130aug23_003plasma MetS-MCI
SA45101229aug23_048plasma MetS-MCI
SA45101329aug23_054plasma MetS-MCI
SA45101429aug23_062plasma MetS-MCI
SA45101529aug23_050plasma MetS-MCI
SA45101629aug23_076plasma MetS-MCI
SA45101730aug23_005plasma MetS-MCI
SA45101829aug23_046plasma MetS-MCI
SA45101929aug23_074plasma MetS-MCI
SA45102029aug23_049plasma MetS-MCI
SA45102129aug23_042plasma MetS-MCI
SA45102229aug23_124plasma MetS-MCI
Showing results 1 to 95 of 95

Collection:

Collection ID:CO004076
Collection Summary:Blood was collected in EDTA-coated tubes and centrifuged at 3,000 rpm to obtain plasma. The plasma was then transferred into cryovials and stored at –85°C until use.
Sample Type:Blood (plasma)

Treatment:

Treatment ID:TR004092
Treatment Summary:All patients were divided into two groups: 1) MetS patients with MCI and 2) MetS patients with normal cognitive function. MCI was determined when the MoCA score was less than 23.

Sample Preparation:

Sampleprep ID:SP004089
Sampleprep Summary:Tryptophan was quantified using a previously published method with minor modifications. In brief, 50 µL of plasma was mixed with 200 µL of methanol containing 100 ng of picolinic acid-d4, used as an internal standard. After a brief vortex, the sample mixture was sonicated for 10 min at room temperature and stored at -20 ºC overnight. The sample was centrifuged at 13,000 rpm at 4 ºC for 15 min. The supernatant was transferred to a new Eppendorf tube and evaporated to dryness using a vacuum concentrator. The sample was resuspended in 100 µL Milli-Q water with 0.1% formic acid, followed by a brief vortex and 10-minute sonication at room temperature. After centrifugation at 13,000 rpm, 4 ºC for 15 min, the supernatant was transfer to an LC vial.

Chromatography:

Chromatography ID:CH004929
Chromatography Summary:Tryptophan was quantified using a previously published method with minor modifications. In brief, 50 µL of plasma was mixed with 200 µL of methanol containing 100 ng of picolinic acid-d4, used as an internal standard. After a brief vortex, the sample mixture was sonicated for 10 min at room temperature and stored at -20 ºC overnight. The sample was centrifuged at 13,000 rpm at 4 ºC for 15 min. The supernatant was transferred to a new Eppendorf tube and evaporated to dryness using a vacuum concentrator. The sample was resuspended in 100 µL Milli-Q water with 0.1% formic acid, followed by a brief vortex and 10-minute sonication at room temperature. After centrifugation at 13,000 rpm, 4 ºC for 15 min, the supernatant was transfer to an LC vial.
Instrument Name:Waters Acquity I-Class
Column Name:Waters ACQUITY UPLC HSS C18 (100 x 2.1mm,1.8um)
Column Temperature:30ºC
Flow Gradient:n/a
Flow Rate:0.3 mL/min
Solvent A:100% Water (Milli-Q); 0.1% Formic acid
Solvent B:100% Acetonitrile; 0.1% Formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006487
Analysis Type:MS
Chromatography ID:CH004929
Num Factors:2
Num Metabolites:1
Units:µmol/dL plasma
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