Summary of Study ST004123
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002591. The data can be accessed directly via it's Project DOI: 10.21228/M8QZ83 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST004123 |
| Study Title | Targeted volatile metabolomics of Artemisia argyi leaves under cadmium stress with synthetic microbial community inoculation |
| Study Type | GC-MS |
| Study Summary | This study examined the effects of a native two-member synthetic microbial community (SynCom) on leaf volatile metabolite production in Artemisia argyi grown under cadmium stress. Plants were cultivated for 60 days in Cd-contaminated soil collected from a mining-impacted site in Hunan Province, China, with SynCom-inoculated and uninoculated treatments. Leaf volatiles were extracted via headspace solid-phase microextraction (HS-SPME) and analyzed by GC-MS in SIM mode. Among the 15 identified metabolite classes, terpenoids (23%), esters (18%), and alcohols (10%) predominated. SynCom treatment significantly increased the abundance of volatiles such as hexanal, geraniol, linalool, camphor, and phenylethanol, as well as essential oil precursors including prenol and isoprenoids. Intermediate compounds in terpenoid biosynthesis (e.g., β-sitosterol, lilac aldehyde D) were elevated, and KEGG enrichment analysis revealed upregulation of secondary metabolite biosynthesis, α-linolenic acid metabolism, and butanoate metabolism. These findings demonstrate that SynCom inoculation enhances essential oil biosynthesis pathways, contributing to both improved stress adaptation and the economic utilization potential of A. argyi in heavy metal–contaminated environments. |
| Institute | Central South University, China |
| Department | School of Metallurgy and Environment |
| Laboratory | Institute of Environmental Engineering |
| Last Name | He |
| First Name | Xiao |
| Address | 932 South Lushan Road |
| hexiao0507@gmail.com | |
| Phone | +86 13805204537 |
| Submit Date | 2025-08-11 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | GC-MS |
| Release Date | 2025-08-25 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002591 |
| Project DOI: | doi: 10.21228/M8QZ83 |
| Project Title: | Metabolomic responses of Artemisia argyi to synthetic microbial community inoculation under cadmium stress |
| Project Type: | Environmental metabolomics |
| Project Summary: | This project aims to investigate the metabolic responses of Artemisia argyi to inoculation with a native synthetic microbial community (SynCom) in cadmium-contaminated soils. We will perform untargeted rhizosphere metabolomics and targeted leaf volatile metabolomics to assess how SynCom inoculation influences primary and secondary metabolic pathways under heavy metal stress. The study will focus on identifying key metabolites and metabolic pathways related to microbial carbon fixation, soil organic carbon accumulation, stress signaling, and essential oil biosynthesis. The findings are expected to provide insights into the mechanisms by which SynCom enhances phytoremediation efficiency, carbon sequestration, and the economic utilization potential of A. argyi in mining-impacted environments. |
| Institute: | Central South University, China |
| Department: | School of Metallurgy and Environment |
| Last Name: | He |
| First Name: | Xiao |
| Address: | No. 932 Lushan South Road, Changsha 410083, P.R. China |
| Email: | hexiao0507@gmail.com |
| Phone: | +86 13805204537 |
| Contributors: | Rui Xu |
Subject:
| Subject ID: | SU004272 |
| Subject Type: | Plant |
| Subject Species: | Artemisia argyi |
| Taxonomy ID: | 42236 |
| Genotype Strain: | NA |
| Age Or Age Range: | 60 days |
Factors:
Subject type: Plant; Subject species: Artemisia argyi (Factor headings shown in green)
| mb_sample_id | local_sample_id | Sample source | Treatment |
|---|---|---|---|
| SA476383 | CK1 | Artemisia argyi leaf | No SynCom |
| SA476384 | CK2 | Artemisia argyi leaf | No SynCom |
| SA476385 | CK3 | Artemisia argyi leaf | No SynCom |
| SA476386 | T1 | Artemisia argyi leaf | SynCom inoculation |
| SA476387 | T2 | Artemisia argyi leaf | SynCom inoculation |
| SA476388 | T3 | Artemisia argyi leaf | SynCom inoculation |
| Showing results 1 to 6 of 6 |
Collection:
| Collection ID: | CO004265 |
| Collection Summary: | Leaves of Artemisia argyi were collected for targeted volatile metabolomics to evaluate the impact of synthetic microbial community (SynCom) inoculation under cadmium stress. Samples were taken from mature, healthy leaves to analyze essential oil composition and related volatile metabolites. |
| Sample Type: | Plant |
| Collection Method: | Fresh leaves were excised using sterile scissors, immediately flash-frozen in liquid nitrogen, and stored at −80 °C until analysis. |
| Collection Location: | Central South University, Changsha, Hunan, China |
| Collection Frequency: | Once at harvest (day 60 after transplanting) |
| Collection Duration: | Approximately 2–3 minutes per plant |
| Volumeoramount Collected: | ~2 g fresh weight per sample |
| Storage Conditions: | -80℃ |
Treatment:
| Treatment ID: | TR004281 |
| Treatment Summary: | Synthetic microbial community (SynCom) inoculation was applied to Artemisia argyi plants grown under cadmium (Cd) stress to evaluate its effects on plant growth, phytoremediation performance, and leaf volatile metabolite composition. |
| Treatment: | Biological treatment (microbial inoculation) + chemical stress (Cd contamination) |
| Treatment Compound: | Synthetic microbial community (SynCom) consisting of multiple rhizosphere bacterial strains; cadmium chloride (CdCl₂) for stress simulation |
| Treatment Route: | Soil drench inoculation for SynCom; Cd contamination via pre-mixed soil amendment |
| Treatment Dose: | SynCom inoculation: 50 mL suspension (OD600=0.8) per pot |
| Plant Growth Location: | Central South University, Changsha, China |
| Plant Light Period: | 16 h light / 8 h dark photoperiod |
| Plant Humidity: | 60 ± 5% RH |
| Plant Temp: | 25 ± 2 °C |
| Plant Watering Regime: | Watered with deionized water every 3 days to maintain ~60% field capacity |
| Plant Nutritional Regime: | No additional fertilizer applied |
| Plant Estab Date: | 2024-04-15 |
| Plant Harvest Date: | 2024-06-15 |
Sample Preparation:
| Sampleprep ID: | SP004278 |
| Sampleprep Summary: | Metabolites were extracted from ground samples (500 mg) placed in 20 mL headspace vials containing NaCl-saturated solution to inhibit enzyme activity. Vials were sealed and incubated at 60 °C for 5 min, followed by exposure of a 120 µm DVB/CWR/PDMS SPME fiber to the headspace for 15 min at 60 °C. After extraction, volatiles were thermally desorbed in the GC injection port at 250 °C for 5 min in splitless mode prior to GC-MS analysis. |
| Sampleprep Protocol Filename: | XH_GC-MS_protocol.pdf |
| Extraction Method: | Thermal desorption in GC injection port at 250°C for 5 min, splitless mode |
Chromatography:
| Chromatography ID: | CH005192 |
| Chromatography Summary: | Gas chromatography using Agilent 8890 GC equipped with a DB-5MS capillary column (30 m × 0.25 mm × 0.25 μm). Helium was used as carrier gas at a flow rate of 1.2 mL/min. Oven temperature program: 40°C (3.5 min), 10°C/min to 100°C, 7°C/min to 180°C, 25°C/min to 280°C, hold for 5 min. |
| Methods Filename: | XH_GC-MS_protocol.pdf |
| Instrument Name: | Agilent 8890 |
| Column Name: | Agilent DB5-MS (30m x 0.25mm, 0.25um) |
| Column Temperature: | Programmed: 40°C (3.5 min), 10°C/min to 100 °C, 7°C/min to 180°C, 25°C/min to 280°C, hold for 5 min |
| Flow Gradient: | N/A |
| Flow Rate: | 1.2 mL/min (Helium) |
| Injection Temperature: | 250°C (splitless mode) |
| Solvent A: | N/A |
| Solvent B: | N/A |
| Chromatography Type: | GC |
| Solvent C: | N/A |
Analysis:
| Analysis ID: | AN006836 |
| Analysis Type: | MS |
| Analysis Protocol File: | XH_GC-MS_protocol.pdf |
| Chromatography ID: | CH005192 |
| Num Factors: | 2 |
| Num Metabolites: | 1613 |
| Units: | peak area |
