Summary of Study ST004148

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002610. The data can be accessed directly via it's Project DOI: 10.21228/M88R80 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST004148
Study TitleSurface Acoustic Wave Hemolysis Assay for Evaluating Stored Red Blood Cells
Study SummaryHere we propose a new platform, the Surface Acoustic Wave Hemolysis Assay (SAW-HA), for on-site evaluation of stored RBCs utilizing SAW Hemolysis Temperature (SAWHT) as a marker for RBC quality. We report SAWHT as a mechanism-dependent reproducible methodology for evaluating stored human RBCs up to 42 days. Our results define unique signatures for SAW hemolysis and metabolic profiles in RBCs from two of the six donors in which high body mass index (BMI) and RBC triglycerides associated with increased susceptibility to hemolysis. Metabolic age of the stored RBCs – a recently appreciated predictor of post-transfusion efficacy – reveal that RBCs from the two low SAWHT units were characterized by disrupted redox control and deficient tryptophan metabolism. Together, these findings indicate the potential of the SAW-HA as a point-of-care analysis for transfusion medicine.
Institute
University of Colorado School of Medicine
DepartmentBiochemistry and Molecular Genetics
LaboratoryD'Alessandro
Last NameAnderson
First NameColin
Address13001 East 17th Place
EmailColin.c.anderson@cuanschutz.edu
Phone303-724-3339
Submit Date2025-08-25
Num Groups2
Total Subjects6
Num Males3
Num Females3
PublicationsLab on a Chip
Raw Data AvailableYes
Raw Data File Type(s)mzXML
Analysis Type DetailLC-MS
Release Date2025-08-27
Release Version1
Colin Anderson Colin Anderson
https://dx.doi.org/10.21228/M88R80
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:

Project:

Project ID:PR002610
Project DOI:doi: 10.21228/M88R80
Project Title:Surface Acoustic Wave Hemolysis Assay for Evaluating Stored Red Blood Cells
Project Type:Primary Research Article
Project Summary:Blood transfusion remains a cornerstone of modern medicine, saving countless lives daily. Yet the quality of transfused blood varies dramatically among donors—a critical factor often overlooked in clinical practice. Rapid , benchtop, and cost-effective methods for evaluating stored red blood cells (RBCs) at the site of transfusion are lacking, with concerns persisting about the association between metabolic signatures of stored RBC quality and transfusion outcomes. Recent studies utilizing metabolomics approaches to evaluate stored erythrocytes find that donor biology (e.g., genetics, age, lifestyle factors) underlies the heterogeneity associated with blood storage and transfusion. The appreciation of donor-intrinsic factors provides opportunities for precision transfusion medicine approaches for the evaluation of storage quality and prediction of transfusion efficacy.
Institute:University of Colorado School of Medicine
Department:Biochemistry and Molecular Genetics
Laboratory:Angelo D'Alessandro
Last Name:Anderson
First Name:Colin
Address:12801 East 17th Avenue, Aurora, CO 80045
Email:Colin.c.anderson@cuanschutz.edu
Phone:303-724-3339
Funding Source:NIH
Publications:Lab on a Chip
Contributors:Meiou Song, Colin C. Anderson, Nakul Sridhar, Julie A. Reisz, Leyla Akh, Yu Gao, Angelo D’Alessandro, Xiaoyun Ding

Subject:

Subject ID:SU004298
Subject Type:Human
Subject Species:Homo sapiens
Taxonomy ID:9606

Factors:

Subject type: Human; Subject species: Homo sapiens (Factor headings shown in green)

mb_sample_id local_sample_id Time Sample source
SA480258CA24_59-3day14 pRBCs
SA480259CA24_59-45day14 pRBCs
SA480260CA24_59-37day14 pRBCs
SA480261CA24_59-30day14 pRBCs
SA480262CA24_59-23day14 pRBCs
SA480263CA24_59-15day14 pRBCs
SA480264CA24_59-21day1 pRBCs
SA480265CA24_59-43day1 pRBCs
SA480266CA24_59-35day1 pRBCs
SA480267CA24_59-1day1 pRBCs
SA480268CA24_59-28day1 pRBCs
SA480269CA24_59-13day1 pRBCs
SA480270CA24_59-16day21 pRBCs
SA480271CA24_59-46day21 pRBCs
SA480272CA24_59-4day21 pRBCs
SA480273CA24_59-24day21 pRBCs
SA480274CA24_59-38day21 pRBCs
SA480275CA24_59-31day21 pRBCs
SA480276CA24_59-47day28 pRBCs
SA480277CA24_59-5day28 pRBCs
SA480278CA24_59-39day28 pRBCs
SA480279CA24_59-32day28 pRBCs
SA480280CA24_59-17day28 pRBCs
SA480281CA24_59-25day28 pRBCs
SA480282CA24_59-40day35 pRBCs
SA480283CA24_59-33day35 pRBCs
SA480284CA24_59-18day35 pRBCs
SA480285CA24_59-48day35 pRBCs
SA480286CA24_59-6day35 pRBCs
SA480287CA24_59-26day35 pRBCs
SA480288CA24_59-49day42 pRBCs
SA480289CA24_59-19day42 pRBCs
SA480290CA24_59-34day42 pRBCs
SA480291CA24_59-41day42 pRBCs
SA480292CA24_59-27day42 pRBCs
SA480293CA24_59-7day42 pRBCs
SA480294CA24_59-2day7 pRBCs
SA480295CA24_59-44day7 pRBCs
SA480296CA24_59-14day7 pRBCs
SA480297CA24_59-22day7 pRBCs
SA480298CA24_59-36day7 pRBCs
SA480299CA24_59-29day7 pRBCs
Showing results 1 to 42 of 42

Collection:

Collection ID:CO004291
Collection Summary:Leukodepleted pRBCs in ACD-A/AS-3 were collected via apheresis at Vitalant Blood Donation Center (Denver, CO, USA) from six healthy donors (3 males, 3 females, aged 35–40) with appropriate informed consent and institutional review board approval as specified in the Vitalant research materials .agreement. RBC units were stored at 1–6°C and aseptically sampled weekly from day 1 to day 42. Each week, 100 µL of RBC units were frozen for future metabolic
Sample Type:Blood (whole)

Treatment:

Treatment ID:TR004307
Treatment Summary:pRBCs were analyzed without additional treatments.

Sample Preparation:

Sampleprep ID:SP004304
Sampleprep Summary:For SAW-HA, EHC, and temperature-matched SAW-HA, RBC were diluted 1:10 in PBS (v/v). For Metabolomics, cold 5:3:2 MeOH:ACN:H2O (v/v/v) solution was added in a 10:1 ratio to 5 µL of stored RBCs . Samples were vortexed vigorously for 30 minutes at 4°C, then centrifuged for 10 minutes at 18,213 rcf.

Chromatography:

Chromatography ID:CH005226
Chromatography Summary:5 Min Negative: After sample randomization, 10 μL of extracts were injected into a Thermo Vanquish UHPLC system (San Jose, CA, USA) and resolved on a Waters Acquity BEH C18 column (2.1 x 100 mm, 1.7 um) at 450 μL/min using a 5 min gradient in negative and positive modes (separate runs). The negative polarity gradient utilized mobile phases: A = water, 10 mM ammonium acetate; B = 50% acetonitrile, 50% methanol, 10 mM ammonium acetate. Solvent gradient: 0-0.5 min 0% B, 0.5-1.1 min 0-100% B, 1.1-2.75 min hold at 100% B, 2.75-3 min 100-0% B, 3-5 min hold at 0% B.
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1 mm, 1.7 μm)
Column Temperature:45°C
Flow Gradient:0-0.5 min 0% B, 0.5-1.1 min 0-100% B, 1.1-2.75 min hold at 100% B, 2.75-3 min 100-0% B, 3-5 min hold at 0% B
Flow Rate:450 μL/min
Solvent A:100% Water; 10 mM Ammonium acetate
Solvent B:50% Acetonitrile/50% Methanol; 10 mM Ammonium acetate
Chromatography Type:Reversed phase
  
Chromatography ID:CH005227
Chromatography Summary:5 Min Positive: After sample randomization, 10 μL of extracts were injected into a Thermo Vanquish UHPLC system (San Jose, CA, USA) and resolved on a Waters Acquity BEH C18 column (2.1 x 100 mm, 1.7 um) at 450 μL/min through a 5 min gradient from 5 to 95% organic solvent B (mobile phases: A = water, 0.1% formic acid; B = acetonitrile, 0.1% formic acid) in positive ion mode. Solvent gradient: 0-0.5 min 5% B, 0.5-1.1 min 5-95% B, 1.1-2.75 min hold at 95% B, 2.75-3 min 95-5% B, 3-5 min hold at 5% B.
Instrument Name:Thermo Vanquish
Column Name:Waters ACQUITY UPLC BEH C18 (100 x 2.1 mm, 1.7 μm)
Column Temperature:45°C
Flow Gradient:0-0.5 min 5% B, 0.5-1.1 min 5-95% B, 1.1-2.75 min hold at 95% B, 2.75-3 min 95-5% B, 3-5 min hold at 5% B
Flow Rate:450 μL/min
Solvent A:100% Water; 0.1% Formic acid
Solvent B:100% Acetonitrile; 0.1% Formic acid
Chromatography Type:Reversed phase

Analysis:

Analysis ID:AN006877
Analysis Type:MS
Chromatography ID:CH005226
Num Factors:7
Num Metabolites:74
Units:A.U.
  
Analysis ID:AN006878
Analysis Type:MS
Chromatography ID:CH005227
Num Factors:7
Num Metabolites:93
Units:A.U.
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