Metadata details for analysis AN000409
Study IDST000258
Analysis IDAN000409
Study TitleMetabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in minimal M9 medium
InstituteMcMaster University
SpeciesSinorhizobium meliloti
Ion_modePOSITIVE
MS typeESI
MS Instrument NameBruker MicrOTOF II
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1200 RR Series II
Chromatography TypeHILIC
Chromatography ColumnPhenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um)
Solvent A100% acetonitrile
Solvent B100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid
Gradient0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A
Flow rate200 ?L/min
Column Temperature40C
Retention time unitsMinutes
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