Metadata details for analysis AN000410 | |
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Study ID | ST000258 |
Analysis ID | AN000410 |
Study Title | Metabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in minimal M9 medium |
Institute | McMaster University |
Species | Sinorhizobium meliloti |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Bruker MicrOTOF II |
MS Instrument Type | QTOF |
Chromatography Instrument Name | Agilent 1200 RR Series II |
Chromatography Type | HILIC |
Chromatography Column | Phenomenex Kinetex HILIC 100A (50 x 2.1 mm,2.6um) |
Solvent A | 100% acetonitrile |
Solvent B | 100% water;10 mM ammonium acetate, adjust to pH 3 with formic acid |
Gradient | 0-0.5 min, 95%A; 0.5-12.5 min, 35%A; 12.5-13.0 min, 35%A; 13.0-14.0 min, 95%A; 14.0-24.0 min, 95%A |
Flow rate | 200 ?L/min |
Column Temperature | 40C |
Retention time units | Minutes |