Metadata details for analysis AN001477
Study IDST000909
Analysis IDAN001477
Study TitleMetabolomics Linking Air Pollution, Obesity and Type 2 Diabetes
InstituteEmory University
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000
Chromatography TypeReversed phase
Chromatography ColumnThermo Higgins C18 (50 x 2.1mm,3um)
Solvent A100% water
Solvent B100% acetonitrile
GradientA= water, B= acetontrile, C= 10mM ammonium acetate in water; 60% A, 35% B, 5% C hold for 0.5 min, linear gradient to 0% A, 95% B, 5% C at 1.5 min, hold for 3 min
Flow rate0.4 mL/min for 1.5 min; linear increase to 0.5 mL/min at 2 min held for 3 min
Column Temperature60C
Retention time unitsSeconds
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