Metadata details for analysis AN001514
Study IDST000923
Analysis IDAN001514
Study TitleLongitudinal Metabolomics of the Human Microbiome in Inflammatory Bowel Disease
InstituteBroad Institute of MIT and Harvard
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameShimadzu Nexera X2
Chromatography TypeHILIC
Chromatography ColumnLuna NH2 (Phenomenex; Torrance,CA)
Solvent A100% water; 20 mM ammonium acetate; mM ammonium hydroxide
Solvent B75% acetonitrile/25% methanol; 10 mM ammonium hydroxide
GradientThe column was eluted with initial conditions of 10% mobile phase A and 90% mobile phase B followed by a 10 min linear gradient to 100% mobile phase A.
Flow rate400 µL/min
Column Temperature
Retention time unitsMinutes
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