Metadata details for analysis AN001684 | |
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Study ID | ST001027 |
Analysis ID | AN001684 |
Study Title | Influence of Data-Processing Strategies on Normalized Lipid Levels using an Open-Source LC-HRMS/MS Lipidomics Workflow |
Institute | University of Florida |
Species | Homo sapiens |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
Chromatography Type | Reversed phase |
Chromatography Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
Gradient | Time (min) C (%) D (%) 0 80 20 1 80 20 3 70 30 4 55 45 6 40 60 8 35 65 10 35 65 15 10 90 17 2 98 18 2 98 19 80 20 23 80 20 |
Flow rate | 0.5 mL/min |
Column Temperature | 50 |
Retention time units | Minutes |