| Metadata details for analysis AN001685 | |
|---|---|
| Study ID | ST001027 |
| Analysis ID | AN001685 |
| Study Title | Influence of Data-Processing Strategies on Normalized Lipid Levels using an Open-Source LC-HRMS/MS Lipidomics Workflow |
| Institute | University of Florida |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity BEH C18 (150 x 2.1mm,1.7um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 90% isopropanol/8% acetonitrile/2% water; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | Time (min) C (%) D (%) 0 80 20 1 80 20 3 70 30 4 55 45 6 40 60 8 35 65 10 35 65 15 10 90 17 2 98 18 2 98 19 80 20 23 80 20 |
| Flow rate | 0.5 mL/min |
| Column Temperature | 50 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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