Metadata details for analysis AN001767 | |
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Study ID | ST001082 |
Analysis ID | AN001767 |
Study Title | Combined NMR and MS Analysis of PC patien serum (part-II) |
Institute | Georgia Institute of Technology |
Species | Homo sapiens |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
Chromatography Type | HILIC |
Chromatography Column | Waters Xbridge BEH HILIC (75 x 2.1mm,2.5um) |
Solvent A | 5% acetonitrile/95% water; 10 mM ammonium formate; 0.05% ammonium hydroxide |
Solvent B | 100% acetonitrile; 0.05% ammonium hydroxide |
Gradient | 0-7.0 min. 63% A; 7.0-7.2 min 5% A with an increased flow rate of 500 µL min-1; 7.2-9.9 min 5% A with a decreased flow rate of 300 µL min-1; and 9.9-10.0 min held at 5% A |
Flow rate | 300 µL min-1 |
Column Temperature | 50 |
Retention time units | Minutes |