Metadata details for analysis AN001855
Study IDST001130
Analysis IDAN001855
Study TitleUrea cycle and 1C/serine metabolism in the prevention of oxygen induced retinopathy
InstituteCole Eye Institute
SpeciesMus musculus
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeHILIC
Chromatography ColumnSeQuant ZIC-HILIC (100 x 2.1mm,3.5um)
Solvent A100% water; 0.1% formic acid
Solvent B100% acetonitrile; 0.08% formic acid
Gradientramped from 80% B to 35% B in 23 minutes, followed by a wash step with 5% B from 25-30 min and then re-equilibration with 80% B from 25-30 min.
Flow rate150 µl/min
Column Temperature20
Retention time unitsMinutes
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