| Metadata details for analysis AN001855 | |
|---|---|
| Study ID | ST001130 |
| Analysis ID | AN001855 |
| Study Title | Urea cycle and 1C/serine metabolism in the prevention of oxygen induced retinopathy |
| Institute | Cole Eye Institute |
| Species | Mus musculus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | SeQuant ZIC-HILIC (100 x 2.1mm,3.5um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.08% formic acid |
| Gradient | ramped from 80% B to 35% B in 23 minutes, followed by a wash step with 5% B from 25-30 min and then re-equilibration with 80% B from 25-30 min. |
| Flow rate | 150 µl/min |
| Column Temperature | 20 |
| Retention time units | Minutes |
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