Metadata details for analysis AN001967
Study IDST001185
Analysis IDAN001967
Study TitleGenetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression
InstituteUniversity of Pittsburgh
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnThermo Fisher Accucore C18 (100 x 2.1mm,5um)
Solvent A50% water/50% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate
Solvent B90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium acetate
GradientThe gradient started at 0%B and increased to 50%B from 2-10 min following a second increase to 95%B from 10-47 min. The gradient was held for 4 min at 95%B before increasing to 100%B at 51 min for a 6 min wash. At 57 min the system was returned to initial conditions to equilibrate before the next injection. Total run time was 60 min.
Flow rate0.2 mL/min
Column Temperature
Retention time unitsMinutes
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