Metadata details for analysis AN002084
Study IDST001255
Analysis IDAN002084
Study TitleImmunomodulatory activity of hyaluronidase is associated with metabolic adaptations during acute inflammation
InstituteSao Paulo University
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameABI Sciex 5600 TripleTOF
MS Instrument TypeQTOF
Chromatography Instrument NameShimadzu Nexera X2
Chromatography TypeReversed phase
Chromatography ColumnAscentis Express C18 (100 x 4.6mm,2.7um)
Solvent A70% water/30% acetonitrile; 0.02% acetic acid, pH 5.8(adjusted with ammonium hydroxide)
Solvent B70% acetonitrile/30% isopropanol
Gradient0 to 2.0 min, 0% B; 2.0 to 5.0 min, 15% B; 5.0 to 8.0 min, 20% B; 8.0 to 11.0 min, 35% B; 11.0 to 15.0 min, 70% B; and 15.0 to 19 min, 100% B. At 19.0 min, the gradient returned to the initial condition of 0% B, and the column was re-equilibrated until 25.0 min.
Flow rate0.6ml/min
Column Temperature
Retention time unitsSeconds
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