Metadata details for analysis AN002095
Study IDST001262
Analysis IDAN002095
Study TitleThe impact of tobacco smoke exposure and environmental exposures on the pulmonary microbiome of critically ill children
InstituteEmory University
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameDionex UltiMate 3000
Chromatography TypeReversed phase
Chromatography ColumnHiggins endcapped C18 stainless steel (50 x 2.1mm,3um),Product #TS-0521-C183; Thermo Accucore C18 guard with holder,Product #17126-014005
Solvent A100% water
Solvent B100% acetonitrile
Gradient
Flow rate0.4 mL/min for 1.5 min; linear increase to 0.5 mL/min at 2 min held for 3 min
Column Temperature60C
Retention time unitsSeconds
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