Metadata details for analysis AN002100
Study IDST001264
Analysis IDAN002100
Study TitleAntibiotics and Dietary Minerals Lipidomics
InstituteUniversity of California, Davis
SpeciesRattus norvegicus
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive HF hybrid Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish Focused UHPLC
Chromatography TypeReversed phase
Chromatography ColumnWaters Acquity CSH C18 (100 x 2.1mm,1.7um)
Solvent A60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate
Solvent B90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate
Gradient0 min 15% (B); 0−2 min 30% (B); 2−2.5 min 48% (B); 2.5−11 min 82% (B); 11−11.5 min 99% (B); 11.5−12 min 99% (B); 12−12.1 min 15% (B); and 12.1−15 min 15% (B)
Flow rate0.6 mL/min
Column Temperature65
Retention time unitsMinutes
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