Metadata details for analysis AN002100 | |
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Study ID | ST001264 |
Analysis ID | AN002100 |
Study Title | Antibiotics and Dietary Minerals Lipidomics |
Institute | University of California, Davis |
Species | Rattus norvegicus |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Vanquish Focused UHPLC |
Chromatography Type | Reversed phase |
Chromatography Column | Waters Acquity CSH C18 (100 x 2.1mm,1.7um) |
Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid; 10 mM ammonium formate |
Gradient | 0 min 15% (B); 0−2 min 30% (B); 2−2.5 min 48% (B); 2.5−11 min 82% (B); 11−11.5 min 99% (B); 11.5−12 min 99% (B); 12−12.1 min 15% (B); and 12.1−15 min 15% (B) |
Flow rate | 0.6 mL/min |
Column Temperature | 65 |
Retention time units | Minutes |