Metadata details for analysis AN002734
Study IDST001676
Analysis IDAN002734
Study TitleLipidomic analysis of CD4+ T-cell subsets (Th1,Th2,Th17 and iTreg cells) (part I)
InstituteUniversity of Turku
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6540 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameWaters Acquity UHPLC UNSPSC 41115709
Chromatography TypeReversed phase
Chromatography ColumnWaters BEH C18 (00mm x 2.1mm,1.7um )
Solvent A100% water; 0.1% formic acid; 1% ammonium acetate
Solvent B50% acetonitrile/50% isopropanol; 0.1% formic acid; 1% ammonium acetate
GradientThe gradient was programmed as follows: 0 to 2 min 35-80% B, 2 to 7 min 80-100 % B, 7 to 14 min 100% B. The column was equilibrated with a 7min period of 35 % B prior to the next run.
Flow rate0.4ml/min
Column Temperature
Retention time unitsMinutes
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