| Metadata details for analysis AN002865 | |
|---|---|
| Study ID | ST001758 |
| Analysis ID | AN002865 |
| Study Title | Metabolomics of mouse cerebral cortical brain tissues comparing GF and CONV-R mice |
| Institute | University of North Carolina at Chapel Hill |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | Reversed phase |
| Chromatography Column | Waters Acquity BEH HSS T3 (100 x 2.1mm,1.8um) |
| Solvent A | 100% water; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | 2% B, 0-1 min; 2% to 15% B, 1-3 min; 15% to 50% B, 3-6 min; 50% to 98% B, 6-7.5 min; 98% B, 7.5-11.5 min; 98% to 2% B, 11.5-11.6 min; 2% B, 11.6-15 min |
| Flow rate | 0.4 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
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