Metadata details for analysis AN002907
Study IDST001792
Analysis IDAN002907
Study TitleEffect of external low-dose rate radiation on mouse biofluid metabolomic signatures (part III)
InstituteGeorgetown University
SpeciesMus musculus
Ion_modePOSITIVE
MS typeESI
MS Instrument NameWaters Synapt G2
MS Instrument TypeQTOF
Chromatography Instrument NameWaters Acquity
Chromatography TypeReversed phase
Chromatography ColumnWaters Acquity BEH C18 (50 x 2.1mm,1.7um)
Solvent A100% water; 0.1% formic acid
Solvent Bsolvent B:100% acetonitrile; 0.1% formic acid solvent C:90% isopropanol/10% acetonitrile; 0.1% formic acid
GradientThe gradient for urine was (solvent A and B) 4.0 min 5% B, 4.0 min 20% B, 5.1 min 95% B, and 1.9 min 5% B at a flow rate of 0.5 ml/min. The gradient for serum was (solvent A, B, and C) 4.0 min 98:2 A:B, 4.0 min 40:60 A:B, 1.5 min 2:98 A:B, 2.0 min 2:98 A:C, 0.5 min 50:50 A:C, and 1.0 min 98:2 A:B at a flow rate of 0.5 ml/min.
Flow rate0.5 ml/min
Column Temperature
Retention time unitsMinutes
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