| Metadata details for analysis AN003083 | |
|---|---|
| Study ID | ST001898 |
| Analysis ID | AN003083 |
| Study Title | Evolution of diapause in the African killifish by remodeling ancient gene regulatory landscape |
| Institute | Stanford University |
| Species | Nothobranchius furzeri;Aphyosemion striatum |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Accucore (150 x 2.1mm,2.6um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid;10 mM ammonium acetate |
| Solvent B | 90% isopropanol/10% acetonitrile; 0.1% formic acid;10 mM ammonium acetate |
| Gradient | The gradient profile used was 30% B for 3 min, 30-43% B over 5 min, 43-50% B over 1 min, 55-90% B over 9 min, 90-99% B over 9 min and 99% B for 5 min. |
| Flow rate | 0.2 mL/min |
| Column Temperature | 30 |
| Retention time units | Minutes |
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