Metadata details for analysis AN003084
Study IDST001898
Analysis IDAN003084
Study TitleEvolution of diapause in the African killifish by remodeling ancient gene regulatory landscape
InstituteStanford University
SpeciesNothobranchius furzeri;Aphyosemion striatum
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000 RS
Chromatography TypeReversed phase
Chromatography ColumnThermo Accucore (150 x 2.1mm,2.6um)
Solvent A60% acetonitrile/40% water; 0.1% formic acid;10 mM ammonium acetate
Solvent B90% isopropanol/10% acetonitrile; 0.1% formic acid;10 mM ammonium acetate
GradientThe gradient profile used was 30% B for 3 min, 30-43% B over 5 min, 43-50% B over 1 min, 55-90% B over 9 min, 90-99% B over 9 min and 99% B for 5 min.
Flow rate0.2 mL/min
Column Temperature30
Retention time unitsMinutes
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