Metadata details for analysis AN003086
Study IDST001899
Analysis IDAN003086
Study TitleSystemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites (part I)
InstituteQIMR Berghofer Medical Research Institute
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameAgilent 6545 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1290 Infinity II
Chromatography TypeHILIC
Chromatography ColumnAgilent Zorbax HILIC Plus RRHD (100 x 2.1mm,1.8um,95Å)
Solvent A95% acetonitrile/5% water; 10 mM ammonium acetate,pH neutral
Solvent B50% acetonitrile/50% water; 10 mM ammonium acetate,pH neutral
GradientTotal method runtime was 12 min with the following gradient for both modes: 0 min (1% eluent B) - 3.5 min (50% B) - 5.5 min (99%B) - 6.5 min (99% B) - 6.7 min (1% B) - 12 min (1% B). Flow rate was set to 0.5 mL/min.
Flow rate0.5 mL/min
Column Temperature40
Retention time unitsMinutes
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