Metadata details for analysis AN003239
Study IDST001987
Analysis IDAN003239
Study TitleTranscriptomic and lipidomic analysis unravels the response of Faecalibacterium prausnitzii to calcium palmitate
InstituteUniversity of Groningen
SpeciesFaecalibacterium prausnitzii
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameBruker maXis Impact qTOF
MS Instrument TypeQTOF
Chromatography Instrument NameWaters Acquity
Chromatography TypeReversed phase
Chromatography ColumnAgilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um)
Solvent A40% water/60% acetonitrile; 0.005% formic acid; 10 mM ammonium formate
Solvent B90% isopropanol/10% acetonitrile; 0.01% formic acid; 10 mM ammonium formate
Gradient0- 2 min from 40 to 43% eluent B, 2-12 min from 50 to 54% eluent B, 12-18 min from 70 to 99% eluent B, and 18-20 min 40% eluent B.
Flow rate0.4 mL/min
Column Temperature55
Retention time unitsSeconds
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