Metadata details for analysis AN003239 | |
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Study ID | ST001987 |
Analysis ID | AN003239 |
Study Title | Transcriptomic and lipidomic analysis unravels the response of Faecalibacterium prausnitzii to calcium palmitate |
Institute | University of Groningen |
Species | Faecalibacterium prausnitzii |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Bruker maXis Impact qTOF |
MS Instrument Type | QTOF |
Chromatography Instrument Name | Waters Acquity |
Chromatography Type | Reversed phase |
Chromatography Column | Agilent Zorbax Eclipse Plus C18 (100 x 2.1mm, 1.8 um) |
Solvent A | 40% water/60% acetonitrile; 0.005% formic acid; 10 mM ammonium formate |
Solvent B | 90% isopropanol/10% acetonitrile; 0.01% formic acid; 10 mM ammonium formate |
Gradient | 0- 2 min from 40 to 43% eluent B, 2-12 min from 50 to 54% eluent B, 12-18 min from 70 to 99% eluent B, and 18-20 min 40% eluent B. |
Flow rate | 0.4 mL/min |
Column Temperature | 55 |
Retention time units | Seconds |