Metadata details for analysis AN003597 | |
---|---|
Study ID | ST002198 |
Analysis ID | AN003597 |
Study Title | Untargeted metabolomics of Pinus pinaster needles under heat and drought stress |
Institute | Universidad de Oviedo |
Species | Pinus pinaster |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Bruker Impact II HD |
MS Instrument Type | QTOF |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
Chromatography Type | Reversed phase |
Chromatography Column | Phenomenex Luna Omega Polar C18 (100 x 2.1 mm,1.7um) |
Solvent A | 100% water; 0.1% formic acid |
Solvent B | 100% acetonitrile; 0.1% formic acid |
Gradient | Gradient elution chromatography was performed starting with 100 % A to 98 % A in 1 min; hold for 9 min, gradient to 60 % A in 21 min, gradient to 45 % A in 5 min; hold for 2 min, gradient to 5 % A in 3 min; hold 5 min, return to initial conditions in 3 min and equilibrate for 5 min (total run time: 53 min) |
Flow rate | 0.1 mL/min |
Column Temperature | 30 ÂșC |
Retention time units | Minutes |