| Metadata details for analysis AN003614 | |
|---|---|
| Study ID | ST002210 |
| Analysis ID | AN003614 |
| Study Title | Revealing the Social Biomarkers of Residual Feed Intake by Using 16s rRNA and LC-MS/MS in Duroc Pig |
| Institute | Sichuan Agricultural University |
| Species | Sus scrofa |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Focus |
| MS Instrument Type | Ion trap |
| Chromatography Instrument Name | Waters 2D UPLC |
| Chromatography Type | HILIC |
| Chromatography Column | Waters Acquity BEH C8 (100 x 2.1mm,1.7um) |
| Solvent A | Pos mode:100% water; 0.1% formic acid, Neg mode:100% water; 10 mM ammonium formate |
| Solvent B | 100% acetonitrile |
| Gradient | 0-1 min, 2% B; 1-9 min, 2%-98% B; 9-12 min, 98% B; 12-12.1 min, 98% B to 2% B; and 12.1-15min, 2% B |
| Flow rate | 0.35 mL/min |
| Column Temperature | |
| Retention time units | Minutes |
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