Metadata details for analysis AN003652
Study IDST002238
Analysis IDAN003652
Study TitleLC-HRMS based plasma metabolomics analysis for biomarker discovery of neuroblastoma: 3-O-methyldopa is a new biomarker of poor prognosis of metastatic disease
InstituteIstituto Giannina Gaslini
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Vanquish
Chromatography TypeReversed phase
Chromatography ColumnWaters Acquity BEH C18 (100 x 2mm,1.7um)
Solvent A100% water; 0.1% formic acid
Solvent B100% acetonitrile; 0.1% formic acid
Gradient99% of A for 0.1 min followed by a linear gradient to 100% of B over 15 min, then kept constant for 5 min, brought back to the initial conditions in 0.5 min, and maintained for 5 min.
Flow rate250 µl/min
Column Temperature
Retention time unitsMinutes
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