| Metadata details for analysis AN003654 | |
|---|---|
| Study ID | ST002238 |
| Analysis ID | AN003654 |
| Study Title | LC-HRMS based plasma metabolomics analysis for biomarker discovery of neuroblastoma: 3-O-methyldopa is a new biomarker of poor prognosis of metastatic disease |
| Institute | Istituto Giannina Gaslini |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Vanquish |
| Chromatography Type | HILIC |
| Chromatography Column | Waters Acquity BEH Amide (150 x 2.1mm,1.7um) |
| Solvent A | 100% water; 5 mM ammonium formate, pH 3 |
| Solvent B | 100% acetonitrile |
| Gradient | 90% of mobile phase B for 0.1 min followed by a linear gradient to 70% of mobile phase A over 15 min, then kept constant for 5 min, brought back to the initial conditions in 0.5 min, and maintained for 9 min. |
| Flow rate | 200 µl/min |
| Column Temperature | |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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