Metadata details for analysis AN003686
Study IDST002257
Analysis IDAN003686
Study TitleLipidomic profiling reveals age-dependent changes in complex plasma membrane lipids that regulate neural stem cell aging (Part 1)
InstituteStanford University
SpeciesMus musculus
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000 RS
Chromatography TypeReversed phase
Chromatography ColumnThermo Accucore C18 (150 x 2.1mm,2.6m)
Solvent A60% acetonitrile/40% water; 0.1% formic acid; 10mM ammonium acetate
Solvent B90% isopropanol/10% acetonitrile; 0.1% formic acid; 10mM ammonium acetate
Gradient30% B for 3min, 30-43% in 2min, 43-55% B in 0.1min, 55-65% in 10min, 65-85% B in 6min, 85-100% B in 2min and 100% B for 5min.
Flow rate0.4ml/min
Column Temperature45
Retention time unitsMinutes
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