Metadata details for analysis AN003852
Study IDST002359
Analysis IDAN003852
Study TitleEffect of hypoxia and reoxygenation on the adult hooded seal brain lipidome
InstituteUniversity of Hamburg
SpeciesCystophora cristata
Ion_modePOSITIVE
MS typeESI
MS Instrument NameBruker Daltonics maXis 3G
MS Instrument TypeQTOF
Chromatography Instrument NameDionex Ultimate 3000 UPLC system (Dionex, Idstein, Germany)
Chromatography TypeReversed phase
Chromatography ColumnPhenomenex Kinetex C18 (150 x 2.1mm,1.7um)
Solvent A100% water
Solvent B25% acetonitrile/75% isopropanol
Gradient55% B (0-2 minutes); 55% to 75% B (2-4 minutes); 75% to 100% B (4-18 minutes); 100% B (18-23 minutes), 55% B (23-24 minutes); 55% B (24-27 minutes)
Flow rate300 µL/min
Column Temperature50°C
Retention time unitsMinutes
  logo