Metadata details for analysis AN003914 | |
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Study ID | ST002402 |
Analysis ID | AN003914 |
Study Title | Four-dimensional trapped ion mobility spectrometry lipidomics for high throughput clinical profiling of human blood samples |
Institute | University Medical Center of Mainz |
Species | Homo sapiens |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Bruker timsTOF |
MS Instrument Type | timsTOF |
Chromatography Instrument Name | Bruker UHPLC |
Chromatography Type | Reversed phase |
Chromatography Column | Phenomenex Luna Omega C18 (100 x 2.1mm,1.6um) |
Solvent A | 50% methanol/50% water; 0.1% formic acid; 7.5 mM ammonium formate, 0.1% triethylamine |
Solvent B | 20% methanol/80% isopropanol; 0.1% formic acid; 7.5 mM ammonium formate; 0.1% triethylamine |
Gradient | The LC method consisted of a 20 min runtime with the following gradient: T0 to T1 – mobile phase A at 60% and mobile phase B at 40% which was then elevated to 90% mobile phase B and 10% mobile phase by T16 and up to 99% mobile phase B by T16.5 and then escalated back to 40% mobile phase B by T20 |
Flow rate | 200 µl/min |
Column Temperature | 45 |
Retention time units | Minutes |