| Metadata details for analysis AN003959 | |
|---|---|
| Study ID | ST002432 |
| Analysis ID | AN003959 |
| Study Title | Metabolic impacts of metformin to seasonal influenza vaccination: a pilot study of drug interaction with the immune response |
| Institute | The Jackson Laboratory for Genomic Medicine |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Orbitrap ID-X Tribrid |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Scientific Transcend Duo LX-2 UHPLC |
| Chromatography Type | HILIC |
| Chromatography Column | Thermo Accucore 150 Amide (50 x 2.1mm, 2.6um) |
| Solvent A | 95% acetonitrile/5% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Solvent B | 50% acetonitrile/50% water; 0.1% acetic acid; 10 mM ammonium acetate |
| Gradient | 0.55 ml/min: 0-0.1 min: 0% B, 0.10-5.0 min: 98% B, and 5 min for cleaning and equilibration of column. |
| Flow rate | 0.55 ml/min |
| Column Temperature | 45 |
| Retention time units | Seconds |