Metadata details for analysis AN003986 | |
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Study ID | ST002446 |
Analysis ID | AN003986 |
Study Title | Untargeted metabolomics of miR-142 WT vs KO CML cells |
Institute | Translational Genomics Research Institute |
Species | Mus musculus |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Fusion Tribrid Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
Chromatography Type | HILIC |
Chromatography Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
Solvent A | 95% water/5% acetonitrile; 10 mM ammonium acetate |
Solvent B | 95% acetonitrile/5% water; 10 mM ammonium acetate |
Gradient | 99% B for 1 min, 99% to 85% for 2 min, 85% to 75% B for 3 min, 75% to 30% B for 3 min, 30% B for 1 min and 5 min column equilibration at 99% B |
Flow rate | 0.4 mL/min |
Column Temperature | 40 |
Retention time units | Minutes |