| Metadata details for analysis AN003987 | |
|---|---|
| Study ID | ST002446 |
| Analysis ID | AN003987 |
| Study Title | Untargeted metabolomics of miR-142 WT vs KO CML cells |
| Institute | Translational Genomics Research Institute |
| Species | Mus musculus |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Fusion Tribrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
| Chromatography Type | HILIC |
| Chromatography Column | Waters ACQUITY UPLC BEH Amide (100 x 2.1mm,1.7um) |
| Solvent A | 95% water/5% acetonitrile; 10 mM ammonium acetate |
| Solvent B | 95% acetonitrile/5% water; 10 mM ammonium acetate |
| Gradient | 99% B for 1 min, 99% to 85% for 2 min, 85% to 75% B for 3 min, 75% to 30% B for 3 min, 30% B for 1 min and 5 min column equilibration at 99% B |
| Flow rate | 0.4 mL/min |
| Column Temperature | 40 |
| Retention time units | Minutes |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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