| Metadata details for analysis AN003992 | |
|---|---|
| Study ID | ST002448 |
| Analysis ID | AN003992 |
| Study Title | Metabolic analysis of primary HPV18-genome containing human foreskin keratinocytes compared to untransfected donor-matched controls. |
| Institute | University of Birmingham, UK |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | |
| MS Instrument Type | |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | HILIC |
| Chromatography Column | Thermo Accucore HILIC (50 x 2.1mm,2.6um) |
| Solvent A | 95% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 50% acetonitrile/50% water; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | gradient elution applied was t=0.0, 1% B; t=1.0, 1% B; t=3.0, 15% B; t=6.0, 50% B; t=9.0, 95% B; t=10.0, 95% B; t=10.5, 1% B; t=14.0, 1% B. All changes were linear (curve = 5) |
| Flow rate | 0.50 mL/min |
| Column Temperature | 35 °C |
| Retention time units | Seconds |
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