| Metadata details for analysis AN003995 | |
|---|---|
| Study ID | ST002448 |
| Analysis ID | AN003995 |
| Study Title | Metabolic analysis of primary HPV18-genome containing human foreskin keratinocytes compared to untransfected donor-matched controls. |
| Institute | University of Birmingham, UK |
| Species | Homo sapiens |
| Ion_mode | NEGATIVE |
| MS type | ESI |
| MS Instrument Name | |
| MS Instrument Type | |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | Reversed phase |
| Chromatography Column | Thermo Hypersil GOLD aQ (100 x 2.1mm,1.9um) |
| Solvent A | 60% acetonitrile/40% water; 0.1% formic acid; 10 mM ammonium formate |
| Solvent B | 85.5% isopropanol/9.5% acetonitrile/5% water; 0.1% formic acid; 10 mM ammonium formate |
| Gradient | gradient elution applied was t=0.0, 20% B; t=0.5, 20% B, t=8.5, 100% B; t=9.5, 100% B; t=11.5, 20% B; t=14.0, 20% B. All changes were linear (curve = 5) |
| Flow rate | 0.40 mL/min |
| Column Temperature | 55 °C |
| Retention time units | Seconds |