Metadata details for analysis AN004029
Study IDST002470
Analysis IDAN004029
Study TitleLinking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human plasma profiling
InstituteBroad Institute of MIT and Harvard
SpeciesHomo sapiens
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameShimadzu Nexera X2
Chromatography TypeHILIC
Chromatography ColumnWaters Atlantis HILIC (150 x 2 mm, 3 μm)
Solvent A100% water; 10 mM ammonium formate; 0.1% formic acid
Solvent B100% acetonitrile; 0.1% formic acid
GradientIsocratically with 5% mobile phase A for 1 minute followed by a linear gradient to 40% mobile phase B over 10 minutes
Flow rate250 µL/min
Column Temperature30C
Retention time unitsMinutes
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