Metadata details for analysis AN004032
Study IDST002470
Analysis IDAN004032
Study TitleLinking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human plasma profiling
InstituteBroad Institute of MIT and Harvard
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameShimadzu Nexera X2
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC BEH C18 (150 x 1.7mm,2.1um)
Solvent A100% water; 0.01% formic acid
Solvent B100% acetonitrile; 0.01% acetic acid
GradientThe column was eluted isocratically at a flow rate of 450 µL/min with 20% mobile phase A for 3 minutes followed by a linear gradient to 100% mobile phase B over 12 minutes.
Flow rate450 µL/min
Column Temperature45C
Retention time unitsMinutes
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