Metadata details for analysis AN004033 | |
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Study ID | ST002471 |
Analysis ID | AN004033 |
Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human stool profiling |
Institute | Broad Institute of MIT and Harvard |
Species | Homo sapiens |
Ion_mode | POSITIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Shimadzu Nexera X2 |
Chromatography Type | HILIC |
Chromatography Column | Waters Atlantis HILIC (150 x 2 mm, 3 µm) |
Solvent A | 100% water; 10 mM ammonium formate; 0.1% formic acid |
Solvent B | 100% acetonitrile; 0.1% formic acid |
Gradient | Isocratically with 5% mobile phase A for 1 minute followed by a linear gradient to 40% mobile phase B over 10 minutes |
Flow rate | 250 µL/min |
Column Temperature | 30C |
Retention time units | Minutes |