| Metadata details for analysis AN004033 | |
|---|---|
| Study ID | ST002471 |
| Analysis ID | AN004033 |
| Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human stool profiling |
| Institute | Broad Institute of MIT and Harvard |
| Species | Homo sapiens |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Shimadzu Nexera X2 |
| Chromatography Type | HILIC |
| Chromatography Column | Waters Atlantis HILIC (150 x 2 mm, 3 µm) |
| Solvent A | 100% water; 10 mM ammonium formate; 0.1% formic acid |
| Solvent B | 100% acetonitrile; 0.1% formic acid |
| Gradient | Isocratically with 5% mobile phase A for 1 minute followed by a linear gradient to 40% mobile phase B over 10 minutes |
| Flow rate | 250 µL/min |
| Column Temperature | 30C |
| Retention time units | Minutes |
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