Metadata details for analysis AN004035
Study IDST002471
Analysis IDAN004035
Study TitleLinking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human stool profiling
InstituteBroad Institute of MIT and Harvard
SpeciesHomo sapiens
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Plus Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameShimadzu Nexera X2
Chromatography TypeHILIC
Chromatography ColumnPhenomenex Luna NH2 (150 x 2.1mm, 3um)
Solvent A100% water; 20 mM ammonium acetate; 20 mM ammonium hydroxide
Solvent B75% acetonitrile/25% methanol; 10 mM ammonium hydroxide
GradientThe column was eluted with initial conditions of 10% mobile phase A and 90% mobile phase B followed by a 10 min linear gradient to 100% mobile phase A.
Flow rate400 µL/min
Column Temperature30C
Retention time unitsMinutes
  logo