Metadata details for analysis AN004036 | |
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Study ID | ST002471 |
Analysis ID | AN004036 |
Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Human stool profiling |
Institute | Broad Institute of MIT and Harvard |
Species | Homo sapiens |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Shimadzu Nexera X2 |
Chromatography Type | Reversed phase |
Chromatography Column | Waters ACQUITY UPLC BEH C18 (150 x 1.7mm,2.1um) |
Solvent A | 100% water; 0.01% formic acid |
Solvent B | 100% acetonitrile; 0.01% acetic acid |
Gradient | The column was eluted isocratically at a flow rate of 450 µL/min with 20% mobile phase A for 3 minutes followed by a linear gradient to 100% mobile phase B over 12 minutes. |
Flow rate | 450 µL/min |
Column Temperature | 45C |
Retention time units | Minutes |