Metadata details for analysis AN004040 | |
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Study ID | ST002473 |
Analysis ID | AN004040 |
Study Title | Linking bacterial metabolites to disease-associated microbes to uncover mechanisms of host-microbial interactions in intestinal inflammation. Veillonella parvula media profiling of IBD drug metabolites |
Institute | Broad Institute of MIT and Harvard |
Species | Veillonella parvula |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Plus Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Shimadzu Nexera X2 |
Chromatography Type | HILIC |
Chromatography Column | Phenomenex Luna NH2 (150 x 2.1mm,3um) |
Solvent A | 100% water; 20 mM ammonium acetate; 20 mM ammonium hydroxide |
Solvent B | 75% acetonitrile/25% methanol; 10 mM ammonium hydroxide |
Gradient | The column was eluted with initial conditions of 10% mobile phase A and 90% mobile phase B followed by a 10 min linear gradient to 100% mobile phase A. |
Flow rate | 400 µL/min |
Column Temperature | 30C |
Retention time units | Minutes |