Metadata details for analysis AN004120
Study IDST002504
Analysis IDAN004120
Study TitleLipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acids
InstituteStanford University
SpeciesCaenorhabditis elegans
Ion_modePOSITIVE
MS typeESI
MS Instrument NameThermo Q Exactive Orbitrap
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Dionex Ultimate 3000 RS
Chromatography TypeReversed phase
Chromatography ColumnThermo Accucore C30 (150 x 2.1mm,2.6um)
Solvent A60% acetonitrile/40% water; 1mM ammonium formate; 0.1% formic acid
Solvent B90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid
GradientThe gradient profile used was 30% B for 3 minutes, 30–43% B over 5 minutes, 43–50% B over 1 minute, 55–90% B over 9 minutes, 90-99% B over 9 minutes and 99% B for 5 minutes.
Flow rate0.2 ml/min
Column Temperature30
Retention time unitsMinutes
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