Metadata details for analysis AN004121 | |
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Study ID | ST002504 |
Analysis ID | AN004121 |
Study Title | Lipid droplets and peroxisomes are co-regulated to drive lifespan extension in response to mono-unsaturated fatty acids |
Institute | Stanford University |
Species | Caenorhabditis elegans |
Ion_mode | NEGATIVE |
MS type | ESI |
MS Instrument Name | Thermo Q Exactive Orbitrap |
MS Instrument Type | Orbitrap |
Chromatography Instrument Name | Thermo Dionex Ultimate 3000 RS |
Chromatography Type | Reversed phase |
Chromatography Column | Thermo Accucore C30 (150 x 2.1mm,2.6um) |
Solvent A | 60% acetonitrile/40% water; 1mM ammonium formate; 0.1% formic acid |
Solvent B | 90% isopropanol/10% acetonitrile; 1mM ammonium formate; 0.1% formic acid |
Gradient | The gradient profile used was 30% B for 3 minutes, 30–43% B over 5 minutes, 43–50% B over 1 minute, 55–90% B over 9 minutes, 90-99% B over 9 minutes and 99% B for 5 minutes. |
Flow rate | 0.2 ml/min |
Column Temperature | 30 |
Retention time units | Minutes |