Metadata details for analysis AN004166
Study IDST002532
Analysis IDAN004166
Study TitleNontargeted metabolomics analysis on kidney tissue treated with cisplatin
InstituteChildren's Hospital of Nanjing Medical University
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameThermo Q Exactive Focus
MS Instrument TypeOrbitrap
Chromatography Instrument NameThermo Ultimate 3000
Chromatography TypeReversed phase
Chromatography ColumnWaters ACQUITY UPLC HSS T3 (150 x 2.1mm,1.8um)
Solvent A0.1% formic acid in water and 0.1% formic acid in acetonitrile
Solvent B5 mM ammonium formate in water and acetonitrile
Gradient0~1 min, 2% B/D; 1~9 min, 2%~50% B/D; 9~12 min, 50%~98% B/D; 12~13.5 min, 98% B/D; 13.5~14 min, 98%~2% B/D; 14~20 min, 2% D-positive model (14~17 min, 2% B-negative model)
Flow rate0.25 mL/min
Column Temperature40
Retention time unitsMinutes
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