Metadata details for analysis AN004230
Study IDST002568
Analysis IDAN004230
Study TitleAssessment of metabolic changes in different animal models of maladaptive right ventricular hypertrophy in chronic pulmonary hypertension by lipidomics and HILIC
InstituteUniversidad CEU San Pablo
SpeciesSus scrofa
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameAgilent 6545 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1290 Infinity II
Chromatography TypeReversed phase
Chromatography ColumnAgilent InfinityLab Poroshell 120 EC-C18 (100 x 3mm,2.7um)
Solvent A90% water/1% methanol, 10 mM ammonium acetate, 0.2 mM ammonium fluoride
Solvent B20% acetonitrile/30% methanol/50% isopropanol, 10 mM ammonium acetate, 0.2 mM ammonium fluoride
Gradient0.0-1.0 min: 70% B, 1.0-3.5 min: 86% B, 3.5-10 min: 86% B, 10.0-11.0 min: 100% B, 11.0-17.0 100% B, 17.0-19.0 min: 70% B
Flow rate0.6 mL/min
Column Temperature50 °C
Retention time unitsMinutes
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