Metadata details for analysis AN004232
Study IDST002568
Analysis IDAN004232
Study TitleAssessment of metabolic changes in different animal models of maladaptive right ventricular hypertrophy in chronic pulmonary hypertension by lipidomics and HILIC
InstituteUniversidad CEU San Pablo
SpeciesSus scrofa
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6545 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1290 Infinity II
Chromatography TypeHILIC
Chromatography ColumnWaters XBridge BEH Amide (100 x 2.1mm,2.5um)
Solvent A100% water, 10 mM ammonium formate ,2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506) and 0.1% formic acid
Solvent Bin 10% water/90% acetonitrile, 10 mM ammonium formate 2.5 μM InfinityLab deactivator additive (Agilent, P-N. 5191-4506)
Gradient0.0-3.0min: 98% B, 3.0-6.0 min: 98% B, 6.0-11.0 min: 70% B, 11.0-12.0 min: 60%, 12.0-16.0 min: 5% B, 16.0-18.0 min: 5% B, 18.0-19.0 min: 98% B and 0.1% formic acid
Flow rate0.25 mL/min
Column Temperature25 ºC
Retention time unitsMinutes
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