Metadata details for analysis AN004233
Study IDST002569
Analysis IDAN004233
Study TitleLipidomic analysis in very-long chain acyl-CoA dehydrogenase null mice
InstituteMontreal Heart Institute
SpeciesMus musculus
Ion_modePOSITIVE
MS typeESI
MS Instrument NameAgilent 6530 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameAgilent 1290 Infinity
Chromatography TypeReversed phase
Chromatography ColumnAgilent ZORBAX Eclipse Plus C18 (100 x 2.1mm,1.8um)
Solvent A100% water; 0.2% formic acid; 10 mM ammonium formate
Solvent B55% ethanol,/35% acetonitrile/10% MTBE, 55:35:10; 0.2% formic acid; 10 mM ammonium formate
Gradient0–2 min, 50% B; 2–11 min, 50–85% B; 11–33 min, 85–94% B; 33–35 min, 94–98% B, 35–38 min; 98–99.5% B; 38–82 min, 98–99.5% B; 82–83 min, 99.5–50% B, plus an equilibration of 8 min
Flow rate0.45 ml/min
Column Temperature40
Retention time unitsMinutes
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