| Metadata details for analysis AN004411 | |
|---|---|
| Study ID | ST002721 |
| Analysis ID | AN004411 |
| Study Title | Repeated Exposure to Wood Smoke Alters Pulmonary Gene and Metabolic Profiles |
| Institute | Emory University |
| Species | Rattus norvegicus |
| Ion_mode | POSITIVE |
| MS type | ESI |
| MS Instrument Name | Thermo Q Exactive HF hybrid Orbitrap |
| MS Instrument Type | Orbitrap |
| Chromatography Instrument Name | Thermo Dionex Ultimate 3000 |
| Chromatography Type | HILIC |
| Chromatography Column | Waters XBridge BEH Amide (50 x 2.1mm,2.5um) |
| Solvent A | 100% water |
| Solvent B | 100% acetonitrile |
| Gradient | A= water, B= acetonitrile, C= 2% formic acid in water; 22.5% A, 75% B, 2.5% C hold for 1.5 min, linear gradient to 77.5% A, 20% B, 2.5% C at 4 min, hold for 1 min |
| Flow rate | 0.35 mL/min for 1.5 min; linear increase to 0.4 mL/min at 4 min, hold for 1 min |
| Column Temperature | 60 |
| Retention time units | Seconds |
- UCSD Metabolomics Workbench, a resource sponsored by the Common Fund of the National Institutes of Health
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