Metadata details for analysis AN004458
Study IDST002748
Analysis IDAN004458
Study TitleHER2 overexpression initiates breast tumorigenesis non-cell autonomously by inducing oxidative stress in the tissue microenvironment
InstituteThe University of Manchester
SpeciesMus musculus
Ion_modeNEGATIVE
MS typeESI
MS Instrument NameAgilent 6600 QTOF
MS Instrument TypeQTOF
Chromatography Instrument NameThermo-Fisher Ultimate 3000 HPLC
Chromatography TypeHILIC
Chromatography ColumnAgilent Poroshell 120 HILIC-Z
Solvent A100% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid
Solvent B85% acetonitrile/15% water; 10 mM ammonium acetate adjusted to pH 9 with ammonium hydroxide and 20 µM medronic acid
Gradientflow rate of 0.25 ml/min, starting at 96 % B for 2 minutes, ramping to 65 % B over 20 min, hold at 65 % B for 2 min, then back to 96 % B
Flow rate0.25 ml/min
Column Temperature40
Retention time unitsSeconds
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